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Fig. 3 | BMC Biology

Fig. 3

From: TDP-43 promotes the formation of neuromuscular synapses through the regulation of Disc-large expression in Drosophila skeletal muscles

Fig. 3

TBPH in the muscle promotes synaptic growth through the regulation of Dlg levels. a qRT-PCR analysis of mRNAs immunoprecipitated by Flag-tagged TBPH (UAS-TBPH/+;Mef2-GAL4/+, IP-TBPH) and its mutant variants TBPHF/L (+/+;UAS-TBPH F/L/Mef2-GAL4, IP-TBPHF/L) in adult thoraxes. The dlg enrichment folds was referred to rpl-11 (negative control); hdac6 has been used as the positive control. n = 3 (biological replicates). Individual data values are provided in Additional file 4. Individual Data Values.xls. b Number of peristaltic waves of Ctrl (w1118), Δtb-GFP (tbphΔ23/tbphΔ23;Mef2-GAL4/UAS-GFP), and Δtb-Dlg (tbphΔ23,UAS-Dlg/tbphΔ23;Mef2-GAL4/+). n = 20. c Climbing assay of Ctrl, Δtb-GFP, and Δtb-Dlg using Mef2-GAL4 at day 2. n = 200. d Evoked neurotransmitter release. Representative EJPs evoked by segmental nerve stimulation of Ctrl, Δtb-GFP, and Δtb-Dlg in muscle fiber 6/7 of A3 in third instar larvae. For each fiber, 15 EPPs following 0.5-Hz stimulation were considered. e Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) in Ctrl, Δtb-GFP, and Δtb-Dlg. f Confocal images of third instar NMJ terminal boutons in muscle 6/7 second segment stained with anti-HRP (in green) in Ctrl, Δtb-GFP, and Δtb-Dlg. g Quantification of branch number. n = 15. h Quantification of the bouton shape. n = 200. i Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) and anti-GluRIIA (in red) in Ctrl, Δtb-GFP, and Δtb-Dlg. j Quantification of GluRIIA intensity normalized on Ctrl. n > 200 boutons. **p < 0.01 and ***p < 0.001 calculated by one-way ANOVA, error bars SEM. Scale bar 10 μm (in e) and 5 μm (in f and i)

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