Fig. 5

Dlg defects are conserved in human cells and motoneurons derived from ALS patients carrying TDP-43 mutations. a Western blot analysis on human neuroblastoma (SH-S5Y5) cell line probed for anti-Dlg, anti-GAPDH, and anti-TDP-43 in siGFP (GFP Ctrl) and siTDP-43 (TDP-43 silenced). The same membrane was probe with the three antibodies and the bands of interest were cropped. Quantification of normalized protein amount was reported below each lane, n = 3 (biological replicates). Individual data values are provided in Additional file 4. Individual Data Values.xls. b Western blot analysis probed for anti-Dlg and anti-GAPDH on human differentiated motoneurons derived from iPSCs of an ALS patient (ALS patient #1 and ALS patient #2) and a healthy control (Ctrl #1 and Ctrl #2). The same membrane was probe with the two antibodies, and the bands of interest were cropped. Quantification of normalized protein amount was reported below each lane, n = 3 (biological replicates). Individual data values are provided in Additional file 4. Data Values.xls. c qRT-PCR analysis of DLG. mRNA from human differentiated motoneurons were retrotranscribed with oligo-dT, and DLG transcripts were normalized on GAPDH (housekeeping) gene. n = 2 (biological replicates; real-time reactions performed in duplicate for each biological replicate). Individual data values are provided in the Additional file 4. Individual Data Values.xls. ****p < 0.0001 calculated by one-way ANOVA, error bars SEM