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Fig. 2 | BMC Biology

Fig. 2

From: Using a chimeric respiratory chain and EPR spectroscopy to determine the origin of semiquinone species previously assigned to mitochondrial complex I

Fig. 2

The effect of oxidase pathway inhibitors on quenching ACMA fluorescence in AOX-SMPs. ACMA fluorescence quenching was measured (excitation at 419 nm, emission at 484 nm) during NADH:O2 turnover in AOX-SMPs. The rate of coupled NADH oxidation in AOX-SMPs was rate-matched as in Additional file 1, Figure S1. Measurements were performed with constant stirring at 32 °C in a buffer containing 10 mM Tris-SO4, 50 mM KCl. In total, 50 μg/mL of SMPs or AOX-SMPs were added to a mixture containing 0.5 μM ACMA and 0.1 μM valinomycin. NADH was added to 500 μM. Turnover was inhibited by addition of antimycin A (2 μM) and/or ascofuranone (2 μM) as indicated

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