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Fig. 3 | BMC Biology

Fig. 3

From: Using a chimeric respiratory chain and EPR spectroscopy to determine the origin of semiquinone species previously assigned to mitochondrial complex I

Fig. 3

X-Band continuous-wave EPR spectra of oxygen-supplemented SMPs reduced with NADH. The sample was reduced by 15 mM NADH (final concentration) to approximately 25 mg/mL SMPs under continuous O2 flow in the absence of any inhibitor. Following mixing, the sample was flash-frozen within 7 s (see “Methods”). All spectra have been normalised by the square root of the microwave power, and both panels are shown at the same relative intensity. Vertical lines show the principle g values for the Fe-S clusters of complex I

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