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Fig. 8 | BMC Biology

Fig. 8

From: Using a chimeric respiratory chain and EPR spectroscopy to determine the origin of semiquinone species previously assigned to mitochondrial complex I

Fig. 8

Analysis of 14N couplings from antimycin A-inhibited AOX-SMPs: Quadrupole coupling parameters indicate the identity of 14N nuclei. a Simulated HYSCORE spectrum of SMPs inhibited with carboxin and AOX-SMPs inhibited with carboxin and antimycin. Simulation of major 14N cross peaks with Aiso = 1.00 MHz, T = 0.08 MHz, κ = 0.8 MHz, η = 0.6 MHz (Antimycin insensitive) and Aiso = 0.54 MHz, T = − 0.2 MHz, κ = 0.425 MHz, η = 0.18 MHz (Antimycin sensitive). 14N cross peaks were simulated as two electron spins each coupled to a single 14N nucleus. See methods (Eq. 3) for quadrupole coupling parameters. b Reported 14N quadruple coupling parameters for protein-bound SQs. The two observed sets of 14N couplings from AOX-SMP are labelled on the basis of their antimycin A sensitivity (both species are carboxin insensitive). b was adapted from [37]

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