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Table 1 Representative kinetic measurements on SMPs and AOX-SMPs. AOX-SMPs were preincubated with antimycin A. For assay details, see “Methods

From: Using a chimeric respiratory chain and EPR spectroscopy to determine the origin of semiquinone species previously assigned to mitochondrial complex I

 NADH:O2 (μmol min− 1 mg− 1)Succinate:NAD+ (RET) (μmol min− 1 mg− 1)Inhibition of NADH oxidation (%)Δp (mV)
−Gram+GramRCR−Gram+GramPiericidin AQuinol oxidationa 
SMPs0.301 ± 0.0080.626 ± 0.0112.08 ± 0.050.092 ± 0.0040.009 ± 0.00198.7 ± 0.0195.8 ± 0.03161.4 ± 0.2
AOX-SMPs0.288 ± 0.0260.360 ± 0.0281.25 ± 0.030.065 ± 0.0040.007 ± 0.00599.2 ± 0.0197.1 ± 0.01157.9 ± 1.6
  1. aQuinol oxidation was inhibited by antimycin A in SMPs, and antimycin A and ascofuranone in AOX-SMPs
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