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Fig. 3 | BMC Biology

Fig. 3

From: Nucleus size and DNA accessibility are linked to the regulation of paraspeckle formation in cellular differentiation

Fig. 3

Paraspeckle formation correlates with the size of nucleus. a Images and quantification of nuclear area (μm2) by DAPI staining (blue) and number of NEAT1_2 foci analyzed by smFISH (red) of representative human adult neonatal fibroblasts that exhibited different sizes (scale bar: 10 μm). b Analysis of the correlation between the number of paraspeckles and nucleus size of 100 human neonatal (black) and adult (gray) fibroblasts. c Analysis of the correlation between the averaged number of paraspeckles and average nucleus size per cell in 24 cell types analyzed in the atlas database represented in Fig. 2b. d Average nucleus size (black) and number of paraspeckles (red; based on Fig. 2b) analyzed during reprogramming of human neonatal fibroblasts. e–h Averaged number of paraspeckles per cell (e, g) based on Fig. 2b, d and average nuclear size (f, h) in mouse (gray) and human (black) MSCs and astrocytes (AC), as well as in adult (gray) and neonatal (black) fibroblasts. Numbers on top are the fold changes between the respective cell types from human and mouse. The numbers in red represent predicted fold changes based on slope of regression line in c. i, j Average number of paraspeckles per cell (i) and average nucleus size (j) of NEAT1−/−, NEAT1ΔpA and WT hESCs in pluripotent condition or differentiated by addition of retinoic acid for 3 days to induce paraspeckle formation [18]. The generation of NEAT1−/− hESCs is outlined in Fig. 5a–d. Nucleus size represents the averaged value of 7–14 images per cell type from two independent experiments with 10–100 cells per image (details in methods). Error bars represent standard error of the mean. r in b, c represents Pearson’s correlation coefficient and dashed line is the linear regression line

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