Skip to main content
Fig. 4 | BMC Biology

Fig. 4

From: Nucleus size and DNA accessibility are linked to the regulation of paraspeckle formation in cellular differentiation

Fig. 4

Treatment with DNA-binding small molecule compounds promotes paraspeckle disassembly. a, b Representative images of NEAT1_2 smFISH after treatment of cells by 2 μM ActD (a), 100 μg/ml Hoechst 33342, 5 μM mithramycin A, and 50 μM α-amanitin (b) in trophoblast progenitors produced by 3-day BMP4 treatment of hESCs. Dashed lines show the locations of the borders of the nuclei. Scale bar: 10 μm. c Analysis of the averaged amount of NEAT1_2 foci following ActD treatment in five different cell types. Images in Additional file 3: Figure S3a. d Analysis of the averaged amount of NEAT1_2 foci in trophoblast progenitors following treatment by the four compounds shown in a, b. e Quantification of γ-H2AX foci (associated with DNA double-strand breaks) in trophoblast progenitors and after addition of DNA-binding compounds. Representative images in Additional file 3: Figure S3c. f Analysis of the averaged amount of NEAT1_2 foci in trophoblast progenitors following 2 h of treatment by the compounds as in a, b and different concentrations of the chemotherapeutic reagents vincristine, etoposide, and flavopiridol. DNA-binding and transcriptional inhibition properties of the compounds are listed in Additional file 3: Figure S3d. Error bars in c, d represent standard error of the mean and standard deviation in e, f. Seven images were analyzed in e, f and 14 in c, d, representing two independent replicates using cells of different passages. **** p < 0.0001 unpaired t-test

Back to article page