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Fig. 1 | BMC Biology

Fig. 1

From: Persistent DNA damage triggers activation of the integrated stress response to promote cell survival under nutrient restriction

Fig. 1

Selective survival advantage of XRCC1 KD cells in nutrient-restricted conditions. Phase-contrast images of Tig-1 cells treated with siControl (a, c) or siXRCC1 (b, d) and grown in a medium containing 5% FCS (a, b) or 1% FCS (c, d). Images are from one representative experiment, with four different fields randomly chosen per condition. Scale bar = 400 μm. e Quantification of relative cell area as shown in ad, normalised to siControl grown at 5% FCS. n = 4. Only statistically significant differences are indicated using asterisks. f Cell cycle analysis of siControl and siXRCC1 cells cultured at 15%, 5%, and 1% FCS, respectively. Shown is the percentage of cells in the respective cell cycle phases. n = 3. Statistically significant differences are indicated using asterisks. g Quantification of the percentage of cells in late apoptosis in siControl and siXRCC1 cells cultured at 15%, 5%, and 1% FCS, respectively. n = 3. *p < 0.05. h Quantification of the percentage of necrotic cells in siControl and siXRCC1 cells cultured at 15%, 5%, and 1% FCS, respectively. n = 3. n.s. not significant

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