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Fig. 2. | BMC Biology

Fig. 2.

From: Integrating evolutionarily novel horns within the deeply conserved insect head

Fig. 2.

Region-specific RNAseq during dorsal head development. aO. taurus male and female head morphology. bO. sagittarius male and female head morphology. c We sequenced dorsal head epidermis of male and female O. taurus pre-pupae (shown as adults in cartoons) by dissecting the dorsal head into six distinct regions, which we represent with six rectangles to designate the LatP, MP, LatA, and MA (left and right samples are treated as replicates). Note that, although the dorsal eye is shown in the cartoons, it was removed from the dorsal head during dissections and is not present in the LatP transcriptome. d Number of differentially expressed genes (P adj < 0.001 and log2FC > 2) present in each region for each major pairwise contrast. e Clustering of differentially expressed genes based on similar patterns of expression among the head regions (depicted here with a head region-specific heatmap). Numbers in parentheses show the number of genes present in each cluster. Overlap between genes with similar patterns in males and females are shown in the center

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