Fig. 2

S10 genomic location does not impact ribosome function in normally growing cells. a The GFP expression and OD_600nm of the indicated gfpmut3⁺ strains were measured along time. The fluorescence mean (± SD) was plotted as a function of the mean (± SD) OD_600nm. Figure shows a representative of 3 independent experiments with 4 biological replicates. The parental gfpmut3⁻ strain is an autoflourescence/light dispersion control. b The indicated gfpmut3⁺ strains in early exponential phase were analyzed by FC. Left panel shows the fluorescence signal frequency distribution of the indicated V. cholerae strains. A gfpmut3⁻ strain was added as negative control. Right panel shows the fluorescence intensity with the 95% confidence interval (CI). Points represent individual biological replicates obtained along at least 2 independent experiments. c Parental and movant strains bearing RLU in the chromosome (Table S1) were grown until early exponential phase. Then, RL activity, represented as RL units (RLU), was measured in three independent biological replicates for each strain. d Parental and derivative strains present similar resistance levels to ribosome-targeted antibiotics. On the right panel, chromosomes are represented as in the previous figure. The encoded antibiotic resistance markers are depicted as boxes: Gm in violet and Cm in green. Their approximate genomic location is shown in each strain. On the right, the MIC (μg/mL) for Cm, Gm, and Er for each depicted strain is shown. e Ribosome profiles for the indicated strains as obtained by AUC. Pie charts quantify polysome, 70s, 50s, and 30 s fractions for the indicated strains