Fig. 4
From: Direct evidence for transport of RNA from the mouse brain to the germline and offspring
Detection of human pre-MIR941-1 in mouse embryos. AAV1-encoded pre-MIR941-1 (MIR941) was injected into one striatal hemisphere of five male mice. After 16 weeks post-injection, MIR941 was detected in injection and contralateral sites, cerebellum, lymph node and vas deferens/epididymis (V/E). Injected male mice were mated between 14 and 16 weeks post-injection with wildtype female mice and evidence of MIR941 was detected in 17 of the 60 embryos (7.5 d.p.c) screened. Rabbit β-globin (rBG) fragment was only found in injection and contralateral sites. Positive control (HEK293), negative control (no template control). *Samples that were positive on the gel and were supported by melt-curve analysis