Fig. 5.

TPM1-deficient hematopoietic progenitor cells yield normal-to-increased quantities of functional megakaryocytes and erythroid cells. a WT and KO HPCs put into MK expansion culture generate equivalent numbers of MKs. Points represent CD41⁺/CD42b⁺ MK percentage multiplied by total cell count, normalized to cell count on day 0. ns, not significant. bTPM1 KO MKs respond appropriately to platelet agonists. WT and KO MKs were incubated with Convulxin (CV) or Thrombin (Thr) at the indicated concentrations, and the percentage of activated MKs (PAC-1⁺/CD41⁺/CD42b⁺) were quantified. *p < 0.05 by ANOVA vs WT. c KO HPCs put into erythroid expansion culture generate more erythroid cells than WT HPCs. Points represent CD235⁺ percentage multiplied by total cell count, normalized to cell count on day 0. **p < 0.01 by ANOVA. d Model in which KO iPSCs yield more HPCs than WT, generating more total blood cells