Fig. 1
Generation of β7-F185A KI mice. a Targeted insertion to the Itgb7 locus of the floxed neo cassette, and the mutated exon 5 (5*) that contains β7-F185A. The targeting vector, the WT Itgb7 locus, the targeted Itgb7 allele containing floxed neo cassette, and the mutated Itgb7 (F185A) allele are shown. Exons are shown as thick lines, as well as long arm (LA) and short arm (SA) of homology are also shown. The loxP-flanked neo cassette is deleted by intercrossing the mutant mouse strains with an EIIa-Cre strain, leaving 1 loxP site. S, SacII; N, NotI; C, ClaI; S’, SaII. b Genotyping and confirmation of deleted neo cassette by PCR. Genomic DNA isolated from tails was used for PCR analyses. PCR bands are shown for WT (WT/WT, 360 bp), heterozygote (KI/WT, 380 and 360 bp), and homozygote (KI/KI, 380 bp) samples. c Sequencing analysis of WT and KI mice. DNA sequencing confirmed a phenylalanine-to-alanine substitution at position 185 of the mouse β7 integrin gene in KI mice