Fig. 3
Impaired adhesion and transmigration of β7-F185A KI lymphocytes. a Cell surface expression of integrins ⍺4 and β7 on splenic lymphocytes from WT, Itgb7+/− (+/−), β7 knock-down (KD), KI, and KO mice. All viable lymphocytes were gated using a combination of forward angle and side scatter to exclude dead cells and debris. And the results were presented as histograms for ⍺4 and β7 expression. The numbers within the table show the specific mean fluorescence intensities of FIB504 (anti-β7) and GK1.5 (anti-α4) mAbs. b Adhesion of WT, +/−, KD, KI, and KO splenic lymphocytes to MAdCAM-1 at 1 dyn/cm2 or 2 dyn/cm2 before and after chemokine stimulation. c, d Transmigration of WT, +/−, KD, KI, and KO splenic lymphocytes toward a serum gradient through MAdCAM-1-coated (c) or ICAM-1-coated (d) permeable insert was examined using a modified Boyden chamber assay with a transwell tissue culture system. ***P < 0.001; ns, not significant. AAAP < 0.001 (Student’s t test in a–d). Data are mean ± s.d. of at least 3 independent experiments (a–d). The asterisk in b indicates the changes of total adherent cells