Fig. 1

Nuclear localization of epithin/PRSS14 intracellular domain. a Epithin/PRSS14 in 427.1.86 cells (427) or epithin/PRSS14-knockdown 427(epiKD) cells were stained green using antibodies against its extracellular C-terminal parts (anti-C) and intracellular parts (anti-N). Nucleus staining with DAPI (blue) is also shown. Scale bars, 20 μm. b The Western blot analyses of epithin/PRSS14 in 427 cells and 427 (epiKD) cells are shown. c The 427 cells transfected with FLAG-EICD were stained using anti-FLAG antibody (green) and DAPI (blue). Scale bars, 10 μm. d Schematic diagram of the assay to validate cleavage of epithin/PRSS14. e The 427 cells were transfected as indicated and deprived of serum before stimulation with 10% FBS, 10 μM PMA, or 10 μM PMA plus 7 mg/ml ecotin for 1 h. Luciferase activity under each condition was normalized against β-galactosidase (β-gal) activity and is shown as a bar graph. Error bars indicate the standard errors of the means (n = 3)