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Fig. 4 | BMC Biology

Fig. 4

From: Unraveling the molecular interactions involved in phase separation of glucocorticoid receptor

Fig. 4

GR condensates formation requires interactions with specific chromatin regions. a Representative images of Drosophila melanogaster S2 and U2OS cells expressing GFP-GR incubated with vehicle (Veh) or Dex and imaged by confocal microscopy with the same microscope settings (Scale bar: 5 μm). The asterisk (*) denotes a significantly different foci density (p < 0.05) with respect to that obtained for S2 cells (S2: ncells = 8; U2OS: ncells = 13). Raw data can be found in Additional file 16: Supplementary Table S4. b U2OS cells expressing GFP-GR were incubated with 100 mM corticosterone (Cort) for 1 h, then washed with PBS five times for 15 min (Cort washout) and finally incubated with Dex for 15 min. Representative images of a cell in the three conditions (Scale bar: 5 μm). The zoomed images correspond to the regions indicated by the dashed squares (Scale bar: 2 μm). Foci that present close positions in Cort and Dex conditions are indicated with the same number (1–5). This experiment was run in seven cells obtaining similar results

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