Fig. 5

GR condensates include Mediator. Representative images of U2OS cells co-expressing GFP-GR and JF549-labeled Halo-Med1 treated with vehicle (Veh) or Dex, incubated with medium supplemented with 100 mM NaCl when indicated and imaged by confocal microscopy (Scale bar: 5 μm). Intensity profiles obtained along the lines indicated with gray lines in merged images. The intensity is represented relative to that determined in the nucleoplasm in each case. Blue and yellow arrows point to Med1 and Med1-GR condensates, respectively. These analyses were performed in 15 (Veh), 25 (Dex), and 5 cells (Dex + NaCl) obtaining similar distributions for each condition