Fig. 4

Loss of ACL/ACS or BCKDH results in predominant hypo-acetylation of nucleo-cytosolic and mitochondrial proteins, respectively. a Volcano plot highlighting differentially acetylated sites of ΔACL/iΔACS compared to RH parasites. Statistically significant differences between parasite lines were determined as outlined in the ‘Material and methods’ section. Unchanged acetylation sites are displayed in black, while sites which are significantly hyper- or hypo-acetylated in ΔACL/iΔACS (≥ 2-fold, n = 3, limma p < 0.01) are displayed in red and blue, respectively. b Hyper- and hypo-acetylated sites in ΔACL/iΔACS parasites were sorted according to their sub-cellular localisation using the hyperplexed Localisation of Organelle Proteins by Isotopic Tagging (hyperLOPIT) data available under https://proteome.shinyapps.io/toxolopittzex/ [27]. c Volcano plot highlighting the differentially acetylated sites in ΔBCKDH compared to RH parasites. Statistically significant differences were determined as outlined in the ‘Material and methods’ section. Unchanged acetylation sites are displayed in black, while sites which are significantly (≥ 2-fold, n = 3, limma p < 0.01) hyper- or hypo-acetylated in ΔBCKDH are displayed in red and blue, respectively. d Hyper- and hypo-acetylated sites in ΔBCKDH parasites were sorted according to their sub-cellular localisation using hyperLOPIT data available under https://proteome.shinyapps.io/toxolopittzex/ [27]. BCKDH, branched-chain α-keto acid dehydrogenase-complex; ACL, ATP citrate lyase; ACS, acetyl-CoA synthetase; PM, plasma membrane; IMC, inner membrane complex; ER, endoplasmic reticulum