Fig. 5

Loss of ACL/ACS causes extensive hypo-acetylation of glycolytic enzymes. a Proteins identified as differentially acetylated in ΔACL/iΔACS parasites were analysed using the GO enrichment R-package topGo to identify the enrichment in biological processes against the total T. gondii genome/proteome. The bubble sizes are proportional to the fold enrichment, ranging from 33-fold (histone acetylation, 1) to 3-fold (organic acid metabolic process, 7). Statistically significant enrichment was assessed by Fisher’s exact test (p value < 0.001). b Scheme highlighting the distribution of differentially acetylated sites on metabolic enzymes of ΔACL/iΔACS parasites. Displayed pathways include the glycolysis/gluconeogenesis, the TCA cycle and the apicoplast resident FASII. Coloured circles on the enzymes represent sites which are either hyper- (red) or hypo-acetylated (blue) in ΔACL/iΔACS. ACL, ATP citrate lyase; ACS, acetyl-CoA synthetase; GO, Gene Ontology; TCA, tricarboxylic acid; FASII, fatty acid synthase II; HK, hexokinase; PGI, phosphoglucose isomerase; PFK, phosphofructokinase; FBA, fructose bis-phosphate aldolase; TIM, triosephosphate isomerase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PGK, phosphoglycerate kinase; PGM, phosphoglycerate mutase; ENO, enolase; PK, pyruvate kinase; LDH, lactate dehydrogenase; PEPCK, phosphoenolpyruvate carboxykinase-1; BCKDH, branched-chain α-keto acid dehydrogenase-complex; E1-E3, BCKDH subunits; CS, citrate synthase; ACN, aconitase; ICDH, isocitrate dehydrogenase; KGDH, α-ketoglutarate dehydrogenase; E1-E3, KGDH subunits; SCS, succinyl coenzyme A synthetase; GDH, glutamate dehydrogenase; SDH, succinate dehydrogenase; FH, fumarate hydratase; MDH, malate dehydrogenase; PDH, pyruvate dehydrogenase complex; E1-E3, PDH subunits; Ac-CoA, acetyl-CoA; ACP, acyl carrier protein; Fab, FAS II subunits; ACP, acyl carrier protein; ACC, acetyl-CoA carboxylase; Pyr, pyruvate; OAA, oxaloacetate; DHAP, dihydroxyacetone phosphate; G3P, glyceraldehyde-3-phosphate