Fig. 7

Loss of ACL/ACS or BCKDH alters the T. gondii gene expression. a Graph showing the number of RNA transcripts and proteins which are ≥ 2-fold up- (red) or downregulated (blue) in ΔACL/iΔACS parasites compared to RH (n = 3; limma p < 0.01). Statistical significance was determined as outlined in the ‘Material and methods’ section. b Venn diagram highlighting the overlap between differentially expressed RNA transcripts (Tra), proteins (Pro) and differentially acetylated proteins (Ace) which present a ≥ 2-fold change in ΔACL/iΔACS parasites compared to RH parasites (all p < 0.01). c Table highlighting genes which were found to be significantly up- (red background) and downregulated (blue background) at the transcriptome and proteome level in ΔACL/iΔACS parasites. d Graph highlighting the number of RNA transcripts and proteins which are ≥ 2-fold up- (red) or downregulated (blue) in ΔBCKDH parasites compared to RH (n = 3; limma p < 0.01). Statistical significance was determined as outlined in the ‘Material and methods’ section. e Venn diagram highlighting the overlap between differentially regulated acetylation sites (Ace), RNA transcripts (Tra) and proteins (Pro), which present a > 2-fold change (p < 0.01) in ΔBCKDH parasites compared to RH parasites. f Table highlighting genes which were found to be significantly up- (red background) and downregulated (blue background) at the transcriptome and proteome level in ΔBCKDH parasites. BCKDH, branched-chain α-keto acid dehydrogenase-complex; ACL, ATP citrate lyase; ACS, acetyl-CoA synthetase; RNA, ribonucleic acid