Fig. 5

NMDA rises global neuronal activity in WT and HD striatal primary cultures. a Experiment timeline. Spontaneous neuronal activity was recorded during 10 min in basal conditions and after addition of 10 μM NMDA + 10 μM glycine. b Percentage of active neurons. c Representative raster plots of spikes and average fluorescence trace before (left) and after (right) addition of NMDA for WT (upper) and HD (lower). d Global activity rate of the cultures and e fraction of independent spikes. Each circle represents a single experiment (STR WT n = 10; STR HD n = 10), thick line the mean, thick shaded area the standard error of the mean, and thin shaded area the standard deviation. Statistical analysis was performed using mixed ANOVA and posterior post hoc test with Bonferroni’s multiple comparison correction. Only * for the post hoc test are shown, *p < 0.05, **p < 0.01, ***p < 0.001