The roles of hybridization and habitat fragmentation in the evolution of Brazil’s enigmatic longwing butterflies, Heliconius nattereri and H. hermathena

Table 1 Heliconius hermathena and H. nattereri genome assembly statistics in comparison with other well-assembled nymphalid genomes

Species^a	Length (Mb)	No. of scaffolds^b	N50 (Mb)	BUSCO^c
Species^a	Length (Mb)	No. of scaffolds^b	N50 (Mb)	Comp. (Dup.)	Frag. (%)	Miss. (%)
Bicyclus anynana	467	2423	0.66	89.6% (0.7%)	3.9	6.5
Danaus plexippus	245	1009	0.73	96.6% (1.9%)	2.3	1.1
Heliconius charithonia^d	316	5162	0.11	94.3% (0.3%)	3.8	1.6
Heliconius erato demophoon	383	194	10.69	85.5% (0.7%)	4.5	10.0
Heliconius erato lativitta	418	142	5.48	73.4% (0.8%)	5.4	21.2
*Heliconius hermathena*	392	1913	0.56	96.0% (0.3%)	2.4	1.6
Heliconius melpomene	275	209	14.31	86.2% (0.4%)	4.1	9.7
*Heliconius nattereri*	276	261	8.84	97.3% (0.5%)	1.2	1.5
Hypolimnas misippus	409	1580	1.01	88.9% (0.3%)	3.8	7.3
Junonia coenia	586	1101	1.57	96.9% (14.7%)	1.4	1.7
Limenitis arthemis	306	737	2.27	81.7% (1.4%)	1.9	16.4
Melitaea cinxia	383	5633	0.12	57.1% (0.2%)	11.8	31.1
Vanessa tameamea	357	1558	2.99	96.3% (0.4%)	2.2	1.5

^aAccessions are shown in the “Materials and methods” section
^bScaffolds ≥ 5 kb
^cCalculated using BUSCO v3 and the Endopterygota database (2440 SCOs) from OrthoDB v9
^dAssembled here, see the “Materials and methods” section

ISSN: 1741-7007