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Fig. 5 | BMC Biology

Fig. 5

From: A chromosome-level assembly of the cat flea genome uncovers rampant gene duplication and genome size plasticity

Fig. 5

The microbiome of EL fleas. a Breakdown of the C. felis (EL fleas) microbiome. Bar at the top graphically depicts the taxonomic distribution of non-flea Illumina reads across Bacteria, viruses, and Archaea. Each group is further classified, with the major taxa (genus level in most cases) and compiled read size (Mb) provided. Taxa with asterisks are AT-rich genomes that were later determined to match to C. felis mitochondrial reads. bWolbachia genome-based phylogeny estimation. Wolbachia supergroups are within gray ellipses. C. felis-associated wolbachiae are within black boxes. Red (wCfeT) and blue (wCfeJ) stars depict the two novel wolbachiae infecting C. felis, with assembly information for each genome provided at right. Inset: color scheme for nematode and arthropod hosts. For tree estimation, see the “Methods” section. Gel image (unaltered) depicts PCR results using 100 ng of flea template DNA (quantified via nanodrop) in separate reactions with gene-specific primers. cwCfeT contains the unique biotin synthesis operon carried by certain obligately host-associated microbes. Schema follows our previous depiction of the unique bio gene order [37], with all proteins drawn to scale (as a reference, wCfeT BioB is 316 aa). Comparisons are made to the bio proteins of Cardinium endosymbiont of Encarsia pergandiella (cEper1, CCM10336-CCM10341) and Wolbachia endosymbiont of Cimex lectularius (wCle, BAP00143-BAP00148). Red shading and numbers indicate the percent identity across pairwise protein alignments (blastp). dwCfeJ contains a CinA/B operon. Comparisons are made to the CidA/B (top, CAQ54390/1) and CinA/B (bottom) operons of Wolbachia endosymbiont of Culex quinquefasciatus Pel (wPip_Pel, CAQ54402/3). Green, CE clan protease; brown, PD-(D/E) XK nuclease. All proteins are drawn to scale (as a reference, wCfeJ CinB is 777 aa). Red shading and numbers indicate the percent identity across pairwise protein alignments (blastp)

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