Fig. 2

Defective thymic egress in RA-GEF DKO mice. a (Top) Representative CD4 and CD8 profiles of the thymus from WT or DKO mice. (Bottom) Percentages of CD4⁺CD8⁺ double-positive (DP), CD4⁺ or CD8⁺ single-positive cells from the thymi of WT or DKO mice (n = 10). *¹P < 0.002, *²P < 0.001 versus corresponding WT cells. b (Top) Representative CD24 and Qa-2 profiles on CD4⁺ or CD8⁺ single-positive cells from the thymus of WT or DKO mice. (Bottom) The numbers of CD24^low and Qa-2^high, CD4⁺ or CD8⁺ single-positive (SP) cells from the thymi of WT or DKO mice (n = 5). *P < 0.001 versus corresponding WT cells. c (Top) Representative CD69 and TCRβ profiles of the thymus from WT or DKO mice. (Bottom) Percentages of TCRβ ^high CD69^low and TCRβ ^high CD69^high cells in thymocytes of WT or DKO mice (n = 5). *P < 0.001 versus corresponding WT cells. d (Left upper) Representative CD4 and CD8 profiles of the thymus from WT or Rap1a and b double knockout (DKO) mice. (Left lower) Percentages of CD4⁺CD8⁺ DP, CD4⁺ or CD8⁺ SP cells from the thymi from WT or Rap1a/b DKO mice (n = 10). *¹P < 0.005, *²P < 0.002 versus corresponding WT cells. (Right upper) Representative CD69 and TCRβ profiles of the thymus from WT or Rap1a/b DKO mice are shown. (Right lower) Percentages of TCRβ ^high CD69^low and TCRβ ^high CD69^high cells from the thymi of WT or Rap1a/b DKO mice (n = 10). *P < 0.001 versus corresponding WT cells. Each bar graph represents the means ± SEM