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Fig. 3. | BMC Biology

Fig. 3.

From: Relationship between oxygen consumption and neuronal activity in a defined neural circuit

Fig. 3.

Correlation between neuronal discharge and ventricular O2 concentration. a Recording of O2 levels in the bath (top black trace) and ventricle (green trace) along with the resting rate (red trace) of superior oblique nerve spike activity illustrated at extended time scales (black traces) for selected periods before (before), after spiking has been blocked by bath-applied (0.5%; pink area) MS-222 (no spike activity), and during recovery (recovery); the O2 consumption (blue trace) was calculated as the difference of concurrent bath and ventricular O2 concentrations. b Boxplot, depicting O2 concentrations in the bath (black) and ventricle in controls (red; N = 31) and after bath-application of 0.05% (green; N = 5) and 0.5% MS-222 (blue; N = 6) indicating significantly increased ventricular O2 levels when the spike activity was blocked (**p < 0.001; ***p < 0.0001; Mann-Whitney U test. c O2 concentration profile (mean ± SEM) of a midline depth track above the floor of the IVth ventricle in controls and after bath-application of 0.05% and 0.5% MS-222. d O2 consumption (upper blue trace), calculated from O2 concentrations of the bath Ringer and ventricle and corresponding superior oblique nerve discharge rate (upper red trace) during and after bath application of 0.05% MS-222 (pink area); lower traces illustrate the O2 consumption (blue) and resting rate (red) during the recovery from the drug effect at an extended time and amplitude scale (gray area from above). e Scatter plot, depicting the dependency of calculated O2 consumption on the resting rate of the superior oblique nerve during recovery from a 0.5% MS-222-induced block of spike activity (N = 6); data from individual preparations are color-coded; each linear regression line has a slope that is significantly different from zero (p < 0.0001); green (r2 = 0.52), blue (r2 = 0.42), red (r2 = 0.40), black (r2 = 0.71), pink (r2 = 0.55), and orange (r2 = 0.69). N, number of preparations

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