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Fig. 1 | BMC Biology

Fig. 1

From: The evolutionarily conserved ESRE stress response network is activated by ROS and mitochondrial damage

Fig. 1

Multiple mitochondrial pathways were activated by mitochondrial-damaging agents. a, c, e Fluorescent images and b, d, f quantification of GFP fluorescence of C. elegans carrying a, b3XESRE::GFP; c, dPhsp-6::GFP; or e, fPtbb-6::GFP reporters that were treated for 10 h with 50 μM rotenone, 3 mM TTFA, 50 μM antimycin A, 10 mM sodium azide, 20 μM CCCP, or vehicle (DMSO). g Fluorescent images of worms with 3XESRE::GFP (most left), Phsp-6::GFP (second from the left), Ptbb-6::GFP (second from the right), or Phsp-16.1::GFP (most right) reared on E. coli containing empty vector (EV) (top) or spg-7(RNAi) (bottom). Representative images are shown; three biological replicates with ~ 400 worms/replicate were analyzed. Error bars represent SEM. p values were determined from one-way ANOVA, followed by Dunnett’s test. All fold changes were normalized to DMSO control. NS not significant, *p < 0.05, **p < 0.01, ***p < 0.001. See Fig. S1 in Additional File 1 for quantification for g

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