Fig. 3

ESRE reporter activation correlates with DHE fluorescence. a Cellular ROS content was assessed based on DHE fluorescence measurement after treatment with vehicle (DMSO) or rotenone with or without 2.5 mM NAC, 25 mM ascorbate (Asc), or ascorbate and NAC combination (NAC/Asc) for 10 h. b Quantification of DHE fluorescence after the treatment with vehicle (DMSO) or CCCP with or without 2.5 mM NAC or 25 mM ascorbate (Asc) for 10 h. c Correlation of ROS level (DHE fluorescence) with reporters’ expression. d Fluorescent images and e quantification of GFP fluorescence of worms carrying 3XESRE::GFP reporter after treatment with vehicle (DMSO) (top) or rotenone (bottom) with or without 5 mM TEMPOL for 10 h. f Quantification of DHE fluorescence under same treatment conditions as in d, e. g Quantification of GFP fluorescence for 3XESRE::GFP reporter after treatment with vehicle (DMSO) or rotenone with or without 10 μM mitoquinol (MQ10) for 10 h. Representative images for d are shown. Three biological replicates with ~ 400 worms/replicate were analyzed. Error bars represent SEM. p values were determined from one-way ANOVA, followed by Dunnett’s test. All fold changes were normalized to DMSO. NS not significant, *p < 0.05, **p < 0.01, ***p < 0.001