Fig. 5
ESRE motif in atfs-1 is necessary for full atfs-1 expression. a Quantification of GFP fluorescence of C. elegans carrying Phsp-6::GFP (left) or Ptbb-6::GFP (right) reporters reared on E. coli expressing empty vector (EV), atfs-1(RNAi), spg-7(RNAi), or spg-7(RNAi); atfs-1(RNAi). b Quantification of GFP fluorescence of Ptbb-6::GFP reporter reared on E. coli expressing empty vector (EV), atfs-1(RNAi), pmk-3(RNAi), or pmk-3(RNAi); atfs-1(RNAi). c Schematic of a mutant in which the ESRE motif located in the atfs-1 promoter was removed (Patfs-1ΔESRE::ATFS-1WT, right), compared to wild type (N2, left). d Quantification of GFP fluorescence of Phsp-6::GFP in two lines of Phsp-6::GFP;Patfs-1ΔESRE::ATFS-1WT reporters reared on E. coli expressing empty vector (EV) or atfs-1(RNAi). Fold changes were normalized to Phsp-6::GFP reared on EV. e Expression of atfs-1 gene in N2 wild type or Patfs-1ΔESRE::ATFS-1WT worms on basal level. Fold change was normalized to N2. f Quantification of GFP fluorescence of Ptbb-6::GFP or Ptbb-6::GFP;Patfs-1ΔESRE::ATFS-1WT reporters reared on E. coli expressing empty vector (EV) or spg-7(RNAi). Fold changes were normalized to Ptbb-6::GFP reared on EV. Three biological replicates with ~ 1000 worms/replicate were analyzed. All fold changes were normalized to EV. Error bars represent SEM. p values were derived from a, b, d, f one-way ANOVA, followed by Dunnett’s test, or et test. NS not significant, *p < 0.05, **p < 0.01, ***p < 0.001