Fig. 6

Human ESRE genes were upregulated by CCCP. Expression of human orthologs of ESRE genes in a bronchial epithelial cells (16HBE) and b human prostate epithelial cells (RWPE-1) after treatment with 20 μM CCCP for 16 h. c Quantification of DHE fluorescence of RWPE-1 cells after treatment with 20 μM CCCP for 16 h. d Expression of human orthologs of ESRE genes in RWPE-1 cells after treatment with 20 μM CCCP alone or in combination with 0.5 mM TEMPOL or 2.5 μM MitoTEMPO for 16 h. Expression levels were normalized to vehicle (DMSO) control. Three biological replicates were analyzed. Error bars represent SEM. p values were determined from a–ct test and d one-way ANOVA, followed by Dunnett’s test. NS not significant, *p < 0.05, **p < 0.01, ***p < 0.001