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Fig. 6 | BMC Biology

Fig. 6

From: The evolution of the Puf superfamily of proteins across the tree of eukaryotes

Fig. 6

Cellular localization of G. intestinalis Puf and Nop9 proteins. a The scores obtained by DeepLoc prediction indicate cytosolic localization of all but GiPUM3 protein, which is predicted as nuclear protein. Colouring gradient represents the values from 0 (red) to 1 (green). Cyt, cytosol; Nuc, nucleus; CM, cytoplasmic membrane; ER, endoplasmic reticulum; Mit, mitochondrion; Extra, extracellular. b Western blot analysis of G. intestinalis expressing BAP-tagged PufSF proteins (one of at least three independent cell experiments is show). SDS-PAGE and immunoblots show total lysate (Lys), cytosolic (Cyt), and high-speed pellet (HSP) fraction. c Immunofluorescence analysis of the same cell lines shows cellular localization of the proteins. PufSF proteins in green, Sec20-endomembrane system marker in red. DIC, Differential Interference Contrast. d Detailed imaging of GiPUM3 by confocal and 2D STED (GiPUM3 in green, DNA in blue). 3D STED of GiPUM3 with the orthogonal projections (GiPUM3 in red)

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