Fig. 1

RTEs are upregulated in TBPH mutants. a Microarray results showing upregulated TEs in TBPH-null mutants: the fold changes are reported for both tbph mutant alleles (Δ23 and Δ142) and referred to w¹¹¹⁸; TE family and class were also indicated. n = 3 (biological replicates). b Real-time quantitative PCR reveals accord and gypsy transcript levels normalized on Rpl11 (housekeeping) in w¹¹¹⁸ - tbph^Δ23,elav-GAL4/tbph^Δ23; UAS-GFP/+ and tbph^Δ23,elav-GAL4/tbph^Δ23,UAS-TBPH. n = 3 (biological replicates, with 3 technical replicates for each), error bars SEM. c Western blot analysis of Drosophila env levels in Δtb-gypsy-IR (tbph^Δ23,elav-GAL4/tbph^Δ23; UAS-gypsy-IR/+), ctrl (tbph^Δ23,elav-GAL4/+), Δtb-GFP-IR (tbph^Δ23,elav-GAL4/tbph^Δ23; UAS-GFP-IR/+), and Δtb-TBPH (tbph^Δ23,elav-GAL4/tbph^Δ23,UAS-TBPH). Adult brains, 1 day old, were probed with anti-ENV and alpha-tubulin antibodies. The same membrane was probed with the two antibodies and the bands of interest were cropped. Quantification of normalized amounts and SD was reported below each lane. n = 2 (biological replicates). *p < 0.05, **p < 0.01, ***p < 0.001 calculated by one-way ANOVA, error bars SEM. Individual data values are provided in Additional file 6: Individual Data Values.pdf