Fig. 5
From: Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model
The GFI axonal projections contain electrical synapse markers. a Giant fiber interneuron (GFI) TRITC-dextran dye injected (magenta, left) and labeled for ShakB (cyan, center) reveals axonal bend projections containing electrical synapses (merge, right) in w1118 (top) and dfmr150M (bottom). Arrowheads indicate ShakB-negative projections; insets show magnified ShakB-positive projections. Scale bars, 10 μm (full image) and 2 μm (inset). b Quantification of total (magenta) and ShakB-positive (cyan) projections for both genotypes. Significance bars represent comparisons between each genotype for the two projection quantifications. Sample sizes: w1118, n = 28; dfmr150M, n = 26. c GFI dye-injected with Neurobiotin (yellow) in shakB2 null mutant alone (left) and the shakB2; dfmr150M double mutant (right). Scale bar, 10 μm. d Quantification of projections for both genotypes. Each gray dot represents the projection number for an axon bend in one animal. The black dot represents the mean and red bars represent the standard error of the mean. Sample sizes: shakB2, n = 27; shakB2; dfmr150M, n = 31