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Fig. 6 | BMC Biology

Fig. 6

From: Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model

Fig. 6

GFI synaptic projections connect within the GF circuit on GFC neurons. a Giant fiber interneuron (GFI) dye co-injected with TRITC-dextran (magenta, left) and neurobiotin (yellow, center) to assay downstream neurons (merge, right) in w1118 (top) and dfmr150M (bottom). Presumed GFC2 (arrow) and GFC3 (arrowhead) are contacted by GFI projections. Scale bar, 10 μm. b GFI injected with TRITC-dextran (magenta) with 73C07-Gal4 driving mCD8::GFP (green) in GFC2 (top), and 24H07-Gal4 driving mCD8::GFP (green) in GFC3 (bottom) in the dfmr150M/+ background. Arrows point to overlaps between GFI and GFCs. Scale bar, 5 μm. c GFI injected with TRITC-dextran (magenta, column 1) with mCD8::GFP (green, column 2) labeling GFC2 (73C07-Gal4, top) and GFC3 (24H07-Gal4, bottom) co-labeled for the ShakB innexin (cyan, column 3) to reveal electrical synapses in the dfmr150M/+ background. All three channels are combined in the merge (column 4). Insets show magnified sub-stacks of ShakB-positive GFI synaptic projections contacting GFC neurons. The arrowhead shows an example ShakB-negative GFI-GFC contact. Scale bars, 5 μm (full image) and 2 μm (inset). All images taken in Airyscan mode for increased resolution

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