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Fig. 2 | BMC Biology

Fig. 2

From: A simple threat-detection strategy in mice

Fig. 2

Flight, freezing, and rearing responses induced by an expanding shadow with constant speed. a The experiment procedure. After 5 min habituation, a looming visual stimulus lasting 6 s was automatically presented when mice entered the trigger area at walking speed; behavioral responses were identified and recorded automatically. b Percentage of flight, freezing, and rearing for each expanding speed for all 1st responses. c Percentage of flight, freezing, and rearing for each expanding speed for all responses. d Example of automatic behavior identification showing a typical flight response and subsequent freezing in the refuge. e Time to reach the refuge after stimulus onset for each expanding speed. f Distance ratio for each expanding speed. g Maximum speed during stimulus for each expanding speed. h Freezing time in the refuge after stimulus onset and before the next entry to the open field for each expanding speed. i Example of automatic behavior identification showing typical freezing behavior in the open field. j Total freezing time in the open field for each expanding speed. k Example of automatic behavior identification showing typical rearing behavior in the trigger area. l Total rearing count in the trigger area for each expanding speed. Each dot represents the result of one looming test trial from one animal. The number of mice and trials for each group is summarized in Table 1. Rank sum tests were calculated for comparisons between the experiment and control groups, and the statistical significance between each pair of groups was corrected using the Bonferroni method. Asterisks indicate the level of statistical significance of the fear indices compared to the negative control group (0°/s), #p < 0.1, *p < 0.05, **p < 0.01, ***p < 0.001

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