Fig. 1

Dengue virus (DENV) replication in wild-type immunocompetent mice treated with interferon receptor blocking antibody. a Four-week- or 10-week-old female C57BL/6 J mice were treated with 1 mg of antibody to block interferon receptor signaling (IFNAR blocking antibody) and then infected with either DENV1 R99142, DENV2 NGC, or DENV2 Puo-218. b Percent weight loss throughout the study following infection. c Viremia following infection as determined by plaque assay in Vero cells. Values are means ± SD from groups of 5–10 animals, except for the mock, which had 2 animals. The studies in 4-week-old mice were repeated only once. The studies in 10-week-old mice were repeated twice, except for the DENV2 NGC groups, which was performed once. Data from the two biological replicates were combined for the 10-week-old mice. No statistical comparisons were made. The dotted line represents the limit of detection (LOD); all negative samples were given a value of 0.5 × LOD for statistical purposes