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Fig. 10 | BMC Biology

Fig. 10

From: Mechanical force-induced morphology changes in a human fungal pathogen

Fig. 10

Filament diameter increases during growth in PDMS and decreases upon bursting out. a Filament diameter inside and outside PDMS at two stiffness values. The diameter of the filament was measured as in Fig. 9a and b (surface and portion inside PDMS). The diameter of the filament adjacent to the mother cell neck in the chamber was measured (portion outside indicated in the schematic). Bars indicate SD, with p < 0.0001 between inside and outside for each PDMS stiffness value (40:1 and 35:1). b Images of filament bursting out of PDMS. Cells grown in PDMS at indicated times over 2 h, with DIC images every 5 min (~ 250 kPa; 30:1) or 10 min (90 kPa; 40:1) shown. c Filament extension rate increases upon exiting PDMS. Filament length was measured from the sum projection GFP images prior to (green and magenta-filled symbols) and following emerging from PDMS (green and magenta-open symbols). d Filament tip diameter is slightly reduced upon emerging from PDMS. The tip diameter was determined at 4–5 times before or after exiting PDMS from b and c. Bars indicate SD and *p = 0.01

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