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Fig. 11 | BMC Biology

Fig. 11

From: Mechanical force-induced morphology changes in a human fungal pathogen

Fig. 11

Invasively growing filaments have increased levels of active Cdc42 at the tip. a, b Tip-localized Cdc42•GTP is increased during invasive growth. a Representative sum projection and DIC images of cells expressing CRIB-GFP on or in 35:1 PDMS (~ 150 kPa). False colored sum projection of 23 × 0.4 μm z-sections (LUT, top) with schematic indicating regions quantitated at the tip and 5–10 μm back from the tip (bottom). b Mean Cdc42•GTP at the tip and 5–10 μm subapically determined from 4 independent time-lapse experiments (images every 5 min for ~ 2 h and sum projections of 23 × 0.4 μm z-sections; n = 16–18 cells). Polarized Cdc42•GTP is the tip signal divided by the subapical signal, 5–10 μm behind the tip (3.5-fold enrichment apically for surface growing cells, normalized to 1). Bars indicate SD and ***p = 0.0002. c, d Active Rho1 is delocalized during invasive growth. c Representative sum projections and DIC images as in a of cells expressing GFP-RID after growth on or in 35:1 PDMS (~ 150 kPa). d Mean Rho1•GTP at the tip and at 5–10 μm subapically was determined from 3 independent time-lapse experiments, as in b (n = 14–20 cells). Polarized Rho1•GTP is the tip signal divided by the subapical signal (1.4-fold enrichment apically for surface growing cells, normalized to 1, as in b). Bars indicate SD and **p = 0.001

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