Fig. 1.
Expression of different enteroendocrine hormones in the zebrafish gut. Whole-mount in situ hybridization (WISH) obtained with different hormonal probes at 3 dpf (a–h, k) or 4 dpf (i, k). Ventral views of embryos with anterior on the left. The dotted lines represent the location of the gut. The probes used are as follows: adcyap1a, adenylate cyclase-activating polypeptide 1a; ccka, cholecystokinin a; gcga, Glucagon a; pyya/b, Peptide YYa/b; ghrl, Ghrelin; gip, glucose-dependent insulinotropic polypeptide; insl5a, insulin-like peptide 5a; calca, calcitonin. Scale bars, 50 μm. i–l Fluorescent in situ hybridization (FISH) (DAPI staining shown in grey)