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Table 1 Identification and expression level of zebrafish enteroendocrine hormones

From: Pancreatic and intestinal endocrine cells in zebrafish share common transcriptomic signatures and regulatory programmes

  Mammalian hormones Zebrafish hormones Synteny Onset time Expression (WISH) Cell number, mean ± SE EEC RNA-seq (CPM) EN RNA-seq (CPM)
Bulb Mid-gut Posterior gut
A 1 Gcg gcga Yes 3.5 dpf 33 ± 1.4 2058 57
gcgb Yes N.T*      177 14
2 Sst sst1.1 Yes   20 13
sst1.2 Yes N.T*      88 1519
sst2 Yes 3 dpf 2.8 ± 0.4 103 933
3 Ghrl ghrl No 3 dpf 2.1 ± 0.4 163 40
4 Pyy pyya Yes 4 dpf   437 1
pyyb Yes 3 dpf 41 ± 2.6 32,011 4
5 Cck ccka Yes 3 dpf 23 ± 3.0 6390 1
6 Gip gip Yes 4 dpf 2.4 ± 0.4 100 4
7 Vip vip Yes N.T*      0 511
vipb No 4 dpf N.T* 927 868
8 Nts nts Yes   0 3
9 Insl5 insl5a No 4 dpf 39 ± 2.2 5602 14
insl5b No N.T*      178 1
10 Nmb nmba Yes N.T*      45 116
nmbb No 3 dpf 14 ± 2.0 2150 0
11 Galn galn Yes 3.5 dpf 26 ± 1.8 824 157
12 Mln / / / / / /   /  
   mlnl No 3 dpf / / 5.9 ± 0.7 708 18
13 Gast / / / / / /   /  
14 Sct / / / / / /   /  
15 Pp / / / / / /   /  
B 16 Adcyap1 adcyap1a No 3 dpf 84 ± 6.9 6927 2
adcyap1b No N.T*      6 251
17 Calca calca Yes 4 dpf 12 ± 0.7 2161 1
18 Penk penka Yes 3 dpf 68 ± 3.6 12,544 2
penkb Yes N.T*      2  
  1. (Upper A panel, lanes 1–15) Identification of the zebrafish orthologues for the 15 mammalian genes coding for enteroendocrine hormones by “in silico” search of the zebrafish genome. (Lower B panel, lanes 16–18) Identification of the 3 novel hormones expressed in zebrafish EECs by screening the RNA-seq data with the Gene Ontology term “hormone activity” (GO: 0005179) and selecting hormones expressed at high levels (> 2.000 CPM). Column 3 indicates the Ensembl ID of the zebrafish genes. Column 4 displays whether a synteny conservation is observed between mouse and zebrafish loci. Column 5 indicates the expression onset time determined by WISH for each hormone transcripts and their localization in the zebrafish gastrointestinal tract (column 6). Bulb corresponds to the enlarged rostral part of the intestine; the mid-gut is located posterior to the bulb and contains the majority of goblet cells at 5 dpf; the posterior gut corresponds the most caudal part of intestine containing few goblet cells [29]. Column 7 indicates the mean (± standard errors (SE)) of the EEC numbers detected by WISH at 4 dpf. The number of EEC counted for each individual larvae is provided in Fig. S2. N.T*: not tested; the number of vipb+ EEC cells could not be quantified as most of the vipb+ cells detected in the gut are vipb+ enteric neurons. Column 8 indicates the expression levels determined by RNA-seq of EEC in CPM (counts per million). Column 9 indicates the expression levels determined by RNA-seq of enteric neurons in CPM [30]. –: not detected by WISH; ✔: detected by WISH
  2. Gcg glucagon, Sst somatostatin, Ghrl ghrelin, Pyy peptide YY, Gast gastrin, Ckk cholecystokinin, Gip glucose-dependent insulinotropic polypeptide, Vip vasoactive intestinal peptide, Mln motilin, mlnl motilin-like, Pp pancreatic polypeptide, Nts neurotensin, Sct secretin, Insl5 insulin-like peptide 5, Nmb neuromedin B, Adcyap1 adenylate cyclase-activating polypeptide 1, Galn galanin, Calca calcitonin
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