Pancreatic and intestinal endocrine cells in zebrafish share common transcriptomic signatures and regulatory programmes

Table 2 Expression level in EECs of transcription factors involved in zebrafish PEC differentiation

Pancreatic endocrine TF	Onset time in the gut	Expression in the gut			EEC RNA-seq (CPM)	EN RNA-seq (CPM)
Pancreatic endocrine TF	Onset time in the gut	Bulb	Mid-gut	Posterior gut	EEC RNA-seq (CPM)	EN RNA-seq (CPM)
ascl1b	–	–	–	–	2	3
ascl1a*	36 hpf	✔	✔	✔	25	55
sox4b*	38 hpf	✔	✔	✔	36	22
insm1b	≤ 52 hpf**	✔	✔	✔	638	189
insm1a	≤ 52 hpf**	N.D	N.D	N.D	123	38
foxo1a	≤ 52 hpf**	✔	✔	✔	504	33
neurod1*	52 hpf	✔	✔	✔	474	67
nkx2.2a*	52 hpf	✔	✔	✔	195	2
pax4	58 hpf	✔	✔	✔	225	0
pax6b*	60 hpf	✔	✔	✔	826	23
rfx6*	62 hpf	✔	–	–	13	8
fev	62 hpf	✔	✔	✔	951	3
isl1*	65 hpf	✔	✔	✔	134	31
arxa	66 hpf	✔	✔	✔	54	1
pdx1	72 hpf in EEC	–	✔	✔	614	4
nkx6.1	–	–	–	–	1	2
nkx6.2	–	–	–	–	5	1
mnx1	–	–	–	–	1	0

Recapitulation of the WISH data performed on zebrafish embryos from 36 to 72 hpf showing the expression onset time and the distribution profile in the gut determined by WISH for 17 transcription factors known to be involved in pancreatic endocrine cell (PEC) differentiation. **Based on RNA-seq data from FACS-sorted sox17:dsRED cells of the microdissected intestine at 52 hpf, insm1a, insm1b and foxo1a are already expressed in the gut at 52 hpf (Reuter et al., article in preparation). The two last columns indicate the expression level in EECs and in enteric neurons (ENs) as determined by RNA-seq in normalized CPM. (N.D.: not determined; −: not detected by WISH; ✔: detected by WISH). All these TF are expressed at significant levels in EEC, except ascl1b, nkx6.1, nkx6.2 and mnx1. Expression data of genes labelled with an asterisk are from [27]

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