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Fig. 3 | BMC Biology

Fig. 3

From: Stability of Begomoviral pathogenicity determinant βC1 is modulated by mutually antagonistic SUMOylation and SIM interactions

Fig. 3

SUMOylation stabilizes βC1 protein in plants. a Stability of transiently overexpressed MBP-βC1 and mK18, 24R SUMOylation-deficient double mutant in N. tabacum. Samples were collected 3 DPI. b Accumulation of transiently overexpressed MBP-βC1 and mK18, 24R upon MG132 treatment. c Quantification of protein levels after MG132 treatment. n = 3. d, e Same as b and c but with NEM treatment. f Stability of transiently overexpressed MBP-tagged βC1 and its SUMOylation motif triple mutant. The number of PCR cycles (cyc) in RT-PCR experiments has been mentioned. Tukey’s multiple comparison test with four stars representing P value, P ≤ 0.0001 and three stars P ≤ 0.001. P, Ponceau staining for total proteins showing RUBISCO large subunit. Other information same as in Fig. 1

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BMC Biology

ISSN: 1741-7007

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