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Fig. 5 | BMC Biology

Fig. 5

From: Stability of Begomoviral pathogenicity determinant βC1 is modulated by mutually antagonistic SUMOylation and SIM interactions

Fig. 5

Mutations in SIM abolished infectivity of βC1. a–d Habit, flower, leaf and height of transgenic GFP-βC1 and its SIM mutants in N. tabacum transgenic lines (n = 3). Photographs and measurements (graphs) are presented. e Stability of βC1 and SIM mutants overexpressing N-terminal GFP-tagged proteins in transgenic plants. Numbers denote individual transgenic lines. f Stability of SIM mutants during transient overexpression. IP of N-terminal GFP-tagged βC1 and mSIM2,3,4 mutants derived from transient overexpression in N. tabacum. g Western blot showing protein levels during transient overexpression of MBP-tagged βC1 and SIM mutants (structural and null mutants) in N. tabacum. h Quantification of g, the y-axis represents protein expression in arbitrary number. n = 3. Marker sizes in kDa are indicated. Size bar in a 10 cm, b top-panel 0.8 cm and bottom panel 2 cm. P, Ponceau staining for total proteins showing RUBISCO large subunit

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BMC Biology

ISSN: 1741-7007

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