Fig. 1

LSTrAP-Crowd pipeline and sample quality control. a LSTrAP-Crowd pipeline. The pipeline is a modification of LSTrAP-Cloud, where the modification allows one group of students to share the task streaming and pseudo-aligning the RNA-seq data. b Scatter plot showing the number of pseudoaligned reads (n_pseudoaligned, x-axis) and % of pseudoaligned reads (p_pseudoaligned, y-axis) of RNA-seq samples for four bacteria. Samples with n_pseudoaligned > 1,000,000 and with high p_pseudoaligned values that were not dissimilar from the majority of samples were used to build the expression matrices. Used samples are indicated by green rectangles