TY - JOUR AU - Liu, Zhiquan AU - Chen, Siyu AU - Shan, Huanhuan AU - Jia, Yingqi AU - Chen, Mao AU - Song, Yuning AU - Lai, Liangxue AU - Li, Zhanjun PY - 2020 DA - 2020/08/31 TI - Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions JO - BMC Biology SP - 111 VL - 18 IS - 1 AB - Cytidine base editors (CBEs), composed of a cytidine deaminase fused to Cas9 nickase (nCas9), enable efficient C-to-T conversion in various organisms. However, current base editors can induce unwanted bystander C-to-T conversions when multiple Cs are present in the ~ 5-nucleotide activity window of cytidine deaminase, which negatively affects their precision. Here, we develop a new base editor which significantly reduces unwanted bystander activities. SN - 1741-7007 UR - https://doi.org/10.1186/s12915-020-00849-6 DO - 10.1186/s12915-020-00849-6 ID - Liu2020 ER -