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Fig. 1 | BMC Biology

Fig. 1

From: Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions

Fig. 1

Base editing with CC context-specificity mediated by eA3G-BE in human cells. a Schematic representation of rA1-BE and eA3G-BE architecture. b Protospacers and PAM (green) sequences of the genomic loci tested, with the target Cs shown in red. Cytosines are counted with the base distal to the PAM setting as position 1. c Summary of C-to-T editing frequencies induced by rA1-BE and eA3G-BE systems on each cytosine at seven target sites. The eA3G-BE showed obvious preference at the cytosines in a CC (red triangle) or CCC (green triangle) context. d Effect of sequence context on base editing by rA1-BE and eA3G-BE (window 4–9). The frequencies were calculated using the data in Fig. 2c. e, f Summary of the base editing frequency at each cytosine in the spacer region for the indicated 7 sgRNAs using rA1-BE (e) and eA3G-BE (f). These data show that the major editing window ranges of rA1-BE and eA3G-BE from positions 4 to 9 or from positions 4 to10 in spacer region, respectively. Values and error bars reflect the mean ± s.e.m. of three independent biological replicates

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