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Fig. 1 | BMC Biology

Fig. 1

From: CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA

Fig. 1

Antisense ribosomal siRNA (risiRNA) accumulats in cde-1 mutant. a NRDE-3 localized to the nucleus in eri-1(mg366);cde-1(tm936);GFP::NRDE-3 animals. Images show representative seam cells of the indicated animals. The percentage of animals with nuclear-enriched NRDE-3 in seam cells is indicated (% N). The number of scored animals is indicated in parentheses. White arrows, nucleus. b The risiRNA sensor was silenced in the cde-1 mutant. (Top) Schematic representation of the risiRNA sensor. (Middle) Images of C. elegans embryos with the indicated genotypes expressing the risiRNA sensor at the ~ 300-cell stage. The levels of GFP expression were scored for each genotype in the bottom panel. c qRT-PCR analysis of risiRNA levels in indicated animals at the L3 stage. Data are presented as the mean ± SD (n = 3, biological replicates). ns, not significant. **P < 0.01 (two-tailed Student’s t test). d, e Deep sequencing of NRDE-3-associated siRNAs in indicated animals. The red dashed line indicates risiRNAs. f The number of risiRNAs targeting the each region of pre-rRNA transcription unit were analyzed

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