Fig. 6

Aneuploid strains show no severe defects in protein biosynthetic capacity. a Biosynthesis of the endogenous protein carboxypeptidase Y (CPY) was monitored following a 5-min pulse-label. All proteins were immunoprecipitated using anti-CPY antibodies and were resolved by SDS-PAGE for wild type (WT) and aneuploid strains. The ER (p1), golgi (p2), and mature vacuolar form (m) of CPY are indicated. Three independent experiments were performed, all yielding consistent observations. Results from one representative experiment are shown. b Average fluorescence of ER-targeted green fluorescent protein (ER-GFP) normalized to steady-state protein levels in WT and aneuploid strains. Error bars represent the standard error of the mean (SEM) of three (D1/2/8) or four (all other strains) independent biological replicates. Welch’s t test: ***p < 0.001, **p < 0.01, *p < 0.05, and not significant p ≥ 0.05 is left unmarked. The following figure supplements are available for Fig. 6: Additional file 14: Fig. S10 and Additional file 15: Table S5