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Fig. 1 | BMC Biology

Fig. 1

From: Independent glial subtypes delay development and extend healthy lifespan upon reduced insulin-PI3K signalling

Fig. 1

Glial Pten over-expression extends lifespan and delays development. a qPCR from head RNA shows over-expression of Pten in 1-week-old wDah;UAS-Pten/+;repo-GAL4/+ flies (red) compared to control wDah;+;repo-GAL4/+ (blue) and wDah;UAS-Pten/+;+ (black) flies. n = 4 replicates of 12 heads per replicate per genotype. b Survival curves show extended lifespan for wDah;UAS-Pten/+;repo-GAL4/+ flies (red) compared to control wDah;+;repo-GAL4/+ (blue) and wDah;UAS-Pten/+;+ (black) flies. n > 150 deaths counted per group. c The number of eggs laid by female flies of the indicated ages was not affected by genotype. n = 15 vials of 12 flies per vial per genotype. d The weight of 1-week-old female flies was not affected by genotype. n = 14 flies per genotype. e Egg-to-adult timing shows delayed development for wDah;UAS-Pten/+;repo-GAL4/+ flies (red) compared to control wDah;+;repo-GAL4/+ (blue) and wDah;UAS-Pten/+;+ (black) flies. n > 200 flies counted per group. f Confocal z-projections show glial nuclei immunostained for REPO (magenta) in the central brain. Dotted lines indicate traces of central brain areas. g Central brain areas were unchanged in 1-week-old wDah;UAS-Pten/+;repo-GAL4/+ flies (red) compared to control wDah;+;repo-GAL4/+ (blue) and wDah;UAS-Pten/+;+ (black) flies. n = 7–8 brains per group. h Confocal z-projections show glial nuclei immunostained for REPO (magenta) and glial membranes labelled with mCD8::GFP (green). Dotted lines indicate areas imaged at higher magnification below. i, j REPO-positive glial nuclei were counted and the extent of glia measured by GFP-positive area in the layer of glia shown in h. Both the density of glial nuclei and the extent of glial membranes were unaffected in 1-week-old wDah;UAS-Pten/+;repo-GAL4/UAS-mCD8::GFP flies (red) compared to control wDah;+;repo-GAL4/UAS-mCD8::GFP (blue) flies. n = 7 brains per group. p values are from Tukey’s multiple comparisons (a), log-rank test versus control group of that colour (b, e), 2-way ANOVA (c), 1-way ANOVA (d, g), or unpaired t-test (i, j)

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