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Fig. 3 | BMC Biology

Fig. 3

From: Independent glial subtypes delay development and extend healthy lifespan upon reduced insulin-PI3K signalling

Fig. 3

Adult-specific IIS inhibition in glia extends lifespan in a foxo-dependent manner. a Confocal z-projections show adult (1-week-old) brains with glial nuclei immunostained for REPO (magenta) and glial membranes labelled with mCD8::GFP (green) in 1-week-old wDah;+;GSG3285-1/UAS-mCD8::GFP flies reared on food containing 200 μM RU-486 or vehicle control from 2 days of age. b qPCR from head RNA shows over-expression of Pten in 2-week-old wDah;UAS-Pten/+;GSG3285-1/+ flies reared on food containing 200 μM RU-486 (induced, red) or vehicle control (uninduced, black) from 2 days of age. n = 6 replicates of 12 heads per replicate per genotype. cf Survival curves show extended lifespan for (c, d) wDah;UAS-Pten/+;GSG3285-1/+, e wDah;UAS-InRDN/+;GSG3285-1/+, and f wDah;UAS-dp110DN/+;GSG3285-1/+ flies reared on food containing 200 μM RU-486 (red curves) from 2 days of age compared to vehicle control (black curves). However, d wDah;UAS-Pten/+;GSG3285-1/+ flies reared on food containing 200 μM RU-486 during larval development alone (blue curve) showed no significant change in lifespan, and flies reared on RU-486 throughout both larval development and adulthood (orange curve) showed no additive lifespan extension from adult-only induction. g Survival curves show extended lifespan for wDah;UAS-Pten/+;GSG3285-1/+ flies (induced, red curve, and uninduced, black curve) and unchanged lifespan for wDah;UAS-Pten/+;GSG3285-1,foxo∆/foxo∆ flies (induced, orange curve, and uninduced, blue curve) reared on food containing 200 μM RU-486 from 2 days of age. h Egg-to-adult timing shows delayed development for wDah;UAS-Pten/+;GSG3285-1/+ larvae (induced, red curve; and uninduced, black curve), and a lesser delay of development for wDah;UAS-Pten/+;GSG3285-1,foxo∆/foxo∆ larvae (induced, orange curve; and uninduced, blue curve), reared on food containing 200 μM RU-486. For all lifespans, n > 140 deaths counted per group; for development assays, n > 40 flies counted per group. p values are from unpaired t test (a), log-rank test versus uninduced (c, e, f), log-rank test for group of that colour versus uninduced (d), or Cox proportional hazards (g, h)

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